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• cDNA libraries and IGF
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Germination
Early growth and tillering
Stem elongation
Wheat apex development
Flowering and fertilisation
Pollen release
Grain growth 1 to 4 days
Grain growth 4 to 10 days
Cell layers inside a grain
Grain filling 11 to 16 days
Grain filling 17 to 21 days
Grain filling 21 to 30 days
Development of the embryo
Dry down 30 to 40 days
How we grew the plants
Photography and microscopy
(days = days after flowering)
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cDNA libraries and IGF
As part of our functional genomics program we have extracted RNA and made cDNA libraries from 35 developmental stages and stress treatments in the wheat variety Mercia. We can use this information to help us learn which genes are switched on and off during the development of wheat grains, and when plants are subjected to stresses such as nitrate starvation, cold and waterlogging. Many of these cDNA libraries correspond to developmental stages shown on this site.
See the cerealsDB site for the latest information on the cDNA libraries: http://www.cerealsdb.uk.net/wheat.htm